Virus x174

The restriction map of a SVphi X recombinant DNA isolate before molecular cloning was indistinguishable from those of two separate cloned derivatives of that isolate, indicating that the species cloned was the major amplifiable recombinant structure generated by a single recombinant-producing cell.

The relative simplicity of the SVphi X recombinant DNA examined is consistent with the notion that most recombinant-producing BSC-1 cells support single recombination events generating only one amplifiable recombinant structure. Full text is available as a scanned copy of the original print version. Get a printable copy PDF file of the complete article 1. Links to PubMed are also available for Selected References. These references are in PubMed.

This may not be the complete list of references from this article. National Center for Biotechnology Information , U. Journal List J Virol v. J Virol. Copyright and License information Disclaimer. Copyright notice. This article has been cited by other articles in PMC. Images in this article Image on p.

Image on p. Brack C. DNA electron microscopy. Quantitation of a simian virus 40 nonhomologous recombination pathway. This leads to the conclusion that phi X binding to cell-bound receptors is irreversible but binding to released receptors is reversible.

The release of virus-receptor complexes from infected cells and the dissociation of these complexes were confirmed by electron microscopy. We propose that initially a single phi X vertex interacts reversibly with E. Abstract At 37 degrees C, binding of phi X to the lipopolysaccharide receptors in the outer membrane of Escherichia coli C is followed by an irreversible ejection of its DNA. Publication types Research Support, Non-U.

Gov't Research Support, U. Gov't, Non-P. Research Support, U.